Infection:Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection.
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Transfection:Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection.
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Western Blot:Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection.
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Quantitative RT-PCR:Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection.
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Cell Adhesion Assay:Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection.
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
CRISPR:Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection.
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Expressing:Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection.
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Software:Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection.
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4˚C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
Article Title: Glaesserella parasuis serotype 4 exploits fibronectin via RlpA for tracheal colonization following porcine circovirus type 2 infection
Article Snippet: Electrophoretically separated proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA), which were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight.were then blocked with 3% bovine serum albumin (BSA) for 2 h. The membranes were then incubated with primary antibodies at 4°C overnight. ... The primary antibodies used were as follows: anti-Fn rabbit polyclonal antibody (1:1000, BA1772) was purchased from BOSTER (Wuhan, China); anti-GFP Mouse mAb (1:5000, M20004) was purchased from Abmart (Shanghai, China); Mouse anti-His-tag antibodies (1:1000, AT0025), Mouse anti-GST-tag antibodies (1:1000, AT0098) and anti-GAPDH mouse mAb (1:5,000; AT0002) were purchased from Engibody Biotechnology (Milwaukee, WI, USA); Smad2 rabbit monoclonal antibody (1:1000, 5339) and phospho-Smad2 rabbit monoclonal antibody (1:1000, 18338) were purchased from Cell Signaling Technology (Beverly, MA, USA).. The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG antibody (1:5000; Cat. No. AT0098, Engibody Biotechnology) at room temperature for 45 min.The membranes were washed with TBST and then incubated with HRP-conjugated goat anti-rabbit (1:5000; Cat. No. AT0097, Engibody Biotechnology) or goat anti-mouse IgG..
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